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KMID : 0360319940260010100
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Journal of Korean Cancer Research Association
1994 Volume.26 No. 1 p.100 ~ p.105
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Mutation of K-ras Oncogene in Non-Small Cell Lung Cancer
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이춘택
강윤구/김창민/조재일/심영목/홍원선/이진오/강태웅
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Abstract
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The mutations of K-ras oncogenes have been detected in about 20~30% of non-small cell lung cancer(NSCLC). In some reports K-ras activations are associated with smoking and poor prognosis in NSCLC patients who undergo curative resection. The ras
oncogenes are usually activated by point mutations. The development of polymerase chain reaction-single strand conformation polymorphism(PCR-SSCP) enables us to detect the subtle nucleotide changes such as point mutations. In SSCP the
electrophoretic
mobility of single strand nucleotide depends on not only its size but also its conformation determined by DNA sequences. We analysed genomic DNAs of 41 human NSCLC obtained by thoracotomy using PCR-SSCP of K-ras codon 12, 13 and K-ras codon 61,
and
compared the results with clinical informations. The electrophoretic mobility changes were found in 10 of 41 NSCLCs(24.4%) in K-ras codon 12, 13. Those changes were found in six of 25 squamous cell carcinomas(24%) and four of 16
adenocarcinomas(25%).
But no changes was found in K-ras codon 61. Comparisons of clinical parameters including age, sex, stage, smoking, and survival showed no significant differences between two groups with or without K-ras mutation. These results suggest that the
mutation
of K-ras oncogene may play an important role in the pathogenesis of some group of NSCLC though the clinical significances of these molecular events are open to further investigations.
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KEYWORD
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